mouse hepatoma cells  (ATCC)

Journal: Toxicological Sciences

Article Title: Functional genomic analysis of non-canonical DNA regulatory elements of the aryl hydrocarbon receptor

doi: 10.1093/toxsci/kfaf146

Figure Lengend Snippet: Mutations in NC-XRE DNA in Serpine1 promoter reduced TCDD-dependent expression of Serpine1 . A) Schematic of mouse Serpine1 gene showing AHR ChIP peak ∼150 bp upstream of transcription start site, which contains 5× repeated NC-XRE (nucleotides in red) and no XRE sequences. Hepa 1 to 6 cell clones containing deletions in the NC-XRE region (dashed lines) are shown below the wild-type sequence. B, C) Hepa 1 to 6 wild-type or mutant cells were exposed to 10 nM TCDD or vehicle for 2 h, followed by RNA extraction and qPCR for Serpine1 (A) or Cyp1a1 (B). Mutant cell lines with full or partial deletion of NC-XRE exhibit reduced increase in Serpine1 following TCDD exposure compared with wild-type cells. Mutant cells showed similar increase in Cyp1a1 following TCDD exposure as wild-type cells. Gene expression normalized to vehicle, 18S rRNA used as reference gene. One-way ANOVA with Tukey’s multiple comparisons test, * P < 0.05, ** P < 0.01, ns, not significant ( P > 0.05).

Article Snippet: The mouse hepatoma cell line Hepa 1 to 6 was obtained from ATCC (Manassas, VA) ( ).

Techniques: Expressing, Clone Assay, Sequencing, Mutagenesis, RNA Extraction, Gene Expression